The Test Subjects are important characters in the game. Most of them are victims of Alfred 's dreadful experiments.
Friendly Test Subjects [ ] These ten are helpful, non-violent individuals who are not a threat to Aya . Four of them are mandatory to help progress the story; four of them are optional and will reward Aya with a Gem upon being helped; and two of them are don't require any help but have varying importance to the overall story.
Aya's Doll [ ] Main Article: Aya's Doll
The first test subject Aya encounters in her adventure.
Cause of death is unknown.
Aya is not aware of the fact that she is a test subject, nor does she ever learn of it.
As her owner is about to enter the Basement 3F Laboratory, she tries to convince Aya to leave Alfred to his fate while hiding in the shadows, but doesn't necessarily push her to do so.
"Didn't you know from the beginning? And yet you still want to help him ? "
Robin [ ] Main Article: Robin
The second test subject Aya encounters in her adventure.
Cause of death was due to Alfred taking away his right eye, but he was quickly revived by the ghost of Monika Drevis .
On Monika's orders, he tries to get Aya to follow him so they can escape from the Drevis Mansion together.
Aya will encounter him at least three times in her adventure.
Stars in his own game mode, "IF Mode", which presents the game's True Ending in his perspective.
"Stay with me. "
Ines [ ] Main Article: Ines
The third test subject Aya encounters.
Cause of death was due to Alfred chopping off her legs.
Aya indirectly meets her at the Basement 1F Storeroom Entrance before meeting her face-to-face at the Basement 3F Left Private Room.
Initially steals Monika's perfume from Aya. Later informs her on how to get past the Creepy Doll.
"Heehee! "
The Mourning Mother [ ] Main Article: Mourning Mother
The fourth test subject Aya encounters in her adventure.
Cause of death is unknown.
Resides at the Mansion 2F Bedroom, where Aya's parents sleep.
Is looking for her daughter, Collina.
Helping her is optional.
Rewards Aya with a Gem once she is reunited with her daughter.
"Give back my daughter... "
Collina [ ] Main Article: Collina
The fifth test subject Aya encounters.
Cause of death was due to Alfred's new medicine he injected into her, which led to her meeting a violent end.
Resides at the Mansion 1F Archives. Appears when Aya examines the "Data of live experimentation " book.
Is looking for her mother.
Gives Aya her pendant, which the young girl needs to lead Collina back to her mother.
Helping her is optional.
"MAMA... MAMA... "
The Headless Man [ ] Main Article: Headless Man
The sixth test subject Aya encounters.
Cause of death appears to be beheading.
Resides at the Basement 1F Mortuary.
Is looking for his body.
Helping him is optional.
Rewards Aya with a Gem if she reunites his head with his body.
"WH-ERE IS BO-DY... "
The Eyeless Girl [ ] Main Article: Eyeless Girl
The seventh test subject Aya encounters.
Cause of death appears to be from having both of her eyes removed.
Resides at the Basement 1F Passage, though her voice is initially heard at the Mansion 1F Reception Room.
Is looking for her eyeballs.
Rewards Aya with a Gem, as well as allowing her to progress further into the basement's depths, upon having her eyeballs returned.
"CAN'T SEE ANYTHING... THAT MAN TOOK AWAY MY EYES! "
The Old Man [ ] Main Article: Old Man
The eighth test subject Aya encounters.
Was initially hired by Monika and Alfred to be a servant for their family, and was not intended to be used as a test subject.
Cause of death was from Monika killing him.
Resides at the Basement 1F Crossroads, where a Gem can be found behind him.
Interacting with him is optional, but he gives some foreshadowing about Monika's true nature.
Has a slightly bigger role in IF Mode.
"That woman 's going to create a demon . She's even crazier than the doctor !
The Mute Subject [ ] Main Article: Mute Subject
The ninth test subject Aya encounters.
Cause of death was from Alfred cutting her arms off with his chainsaw.
Resides at the Basement 1F Stairs to 2F.
Initially prevents Aya from entering the Basement 2F.
Is briefly playable during her flashback.
Rewards Aya by allowing her to progress after witnessing her flashback.
"... "
The Hungry Boy [ ] Main Article: Hungry Boy
The tenth test subject Aya encounters.
Cause of death was starvation and neglect.
Resides at the Basement 2F Cafeteria.
Helping him is optional.
Rewards Aya with a Gem after he is given his favorite food to eat.
"So... hungry. "
